The hydrodynamic size of the nano-probe was 87.4☐.7 nm. The final concentration of protein was calculated to be at 0.78 μgprotein/ml. Results: As results showed, SPIONs-C595 provided active targeting of breast cancer cell (MCF-7) at a final concentration of 600 μgFe/ml. T 2-weighted MR imaging was performed to evaluate the signal enhancement on T 2 relaxation time of nanoprobe using spin-echo pulse sequence. MTT assay was performed to evaluate the cytotoxicity of the nanoprobe in breast cancer cell line (MCF-7). Protein and iron concentration of nanoprobe was examined by standard method. Methods: Nanoprobe characterization was confirmed using Fourier Transform Infra-red Spectroscopy (FT-IR), Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy (SEM-EDAX), and Photon Correlation Spectroscopy (PCS). Modified EDC coupling fraction was used to fabricate the SPION-C595 as an MR imaging contrast agent for breast cancer detection in early stages. Among them, Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) have received particular attention. Background: Advances of nanotechnology have led to the development of nano-materials with both potential diagnostic and therapeutic applications.
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